By Nathan O. Kaplan, Sidney P. Colowick, C. H.W. Hirs, Serge N. Timasheff
The significantly acclaimed laboratory commonplace, Methods in Enzymology, is among the such a lot hugely revered guides within the box of biochemistry. on account that 1955, every one quantity has been eagerly awaited, often consulted, and praised through researchers and reviewers alike. The sequence comprises a lot fabric nonetheless appropriate this day - actually a necessary book for researchers in all fields of existence sciences
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Additional info for Enzyme Structure, Part B
A. Morton, Biochem. J. 40, 628 (1946). *J. R. Spies and D. C. Chambers,Anal. Chem. 21, 1249 (1959). 4T. E. Barman and D. E. , Y. Biol. Chem. 242, 5771 (1967). 5A. Dr~ze, Bull. Soc. Chim. Biol. 42, 407 (1960). eIt. Matsubara and R. M. Sasaki, Biochem. Biophys. Res. Commun. 35, 175 (1969). 44 AMINO ACID ANALYSIS AND RELATED PROCEDURES  prior to derivatization. Most biological samples require a separation of amino acids from interfering substances on a cation-exchange resin. Some samples such as urine require a clean-up on both cation and anionexchange resins.
Kvenvolden, J. G. Lawless, and C. Ponnamperuma, Proc. Nat. Acad. Sci. S. 68, 486 (1971). 2A convenient source of references is the bibliography section of Journal o] Chromatography. 8p. Edman, in "Protein Sequence Determination" (S. B. ), p. 211. Springer-Verlag, Berlin and New York, 1970. 28 AMINO ACID ANALYSISAND RELATED PROCEDURES  power, sensitivity, speed of analysis, and ease of quantitation. 5 In order to realize the full potential of the method, some attention to detail is required, particularly in the preparation of the support and the selection of the proper stationary phases.
Gehrke, K. Kuo, and R. W. Zumwalt, J. Chromatogr. 57, 209 (1971). n D. Roach and C. W. Gehrke, J. Chromatogr. 44, 269 (1969).  CHROMXTOGRAPaV (GLC) OF AmNO XCID DEmVXTIVES 41 but without comment on the extent of its oxidation during derivatization. Usually no more than a 50 M excess of TFAA is used. Greater quantities are likely to interfere in the chromatography because TFAA elutes before all the amino acids, and it tails badly27 Precautions Long experience in the preparation of N-TFA n-butyl esters has led to specific recommendations that must be followed to make the method applicable to all the amino acids?
Enzyme Structure, Part B by Nathan O. Kaplan, Sidney P. Colowick, C. H.W. Hirs, Serge N. Timasheff