Clinical Chemistry

Get Electrochemical methods. Fundamentals and applications PDF

By Allen J. Bard, Larry R. Faulkner

ISBN-10: 0471043729

ISBN-13: 9780471043720

This variation is absolutely revised to mirror the present country off the sector.

  • Significant additions comprise ultramicroelectrodes, changed electrodes, and scanning probe methods.
  • Many chapters were transformed and superior, together with electrode kinetics, voltammetric tools, and mechanisms of coupled chemical reactions.

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Extra resources for Electrochemical methods. Fundamentals and applications

Sample text

This measures @-carotenetogether with any a-or neo-@-carotenesthat may be present. C. COMMENTS Columns prepared from the first three lots of aluminum oxide tested required 45 ml. of eluant to remove the carotene, while columns prepared from the fourth lot tested required 65 ml. The 28 E. M. BICKOFF alumina must be protected against adsorption of moisture to keep its activity constant. The method can also be used to separate carotene from other pigments in a petroleum ether solution that is free of oil.

ASSAY PROCEDURE Five grams of alfalfa meal is soaked in 20 ml. of iso-octane (2,2,4trimethyl pentane) for 15 minutes in the dark. The meal is removed by coarse filtration and the extract transferred to a chromatographic column 12 mm. diameter x 70 mm. in height, packed with a 1 : 1 mixture of magnesia (Westvaco No. 2642) and filter aid. This step need not be quantitative. The column is washed with 100 ml. isooctane which is discarded. Carotene is eluted with 25 ml. of 1 :9 acetone-iso-octane mixture.

Wide and about 15 cm. tall, constricted a t one end and plugged with a wad of cotton. m. of carotene. Wash with the petroleum ether and determine the carotene in the atrate. If the MgCOa is too retentive of carotene, try another lot. Kieselguhr. Wilkes (87) found that heat-treated diatomaceous earths, which have been widely used as filter aids and diluents in preparing adsorbents for chromatographic purposes, possess in themselves a large number of the desired properties of adsorbents for the chromatographic determination of carotene.

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Electrochemical methods. Fundamentals and applications by Allen J. Bard, Larry R. Faulkner


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