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Get Colloid and Interface Science. Biocolloids, Polymers, PDF

By Milton Kerker

ISBN-10: 0124045057

ISBN-13: 9780124045057

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The magnitude of the change, however, is consis­ tent with that which we observed in unfractionated samples of normal and sickling erythrocytes. Experiments to characterize this Fraction III, with emphasis on the age of the cells which make up the population and their polyamine content, are currently underway in our laboratory. VI. THE APPARENT INTERFACIAL VISCOSITY OF THE SICKLING ERYTHROCYTE An interfacial viscometer was constructed in our labora­ tory based on a modification of the design of Brown et_ al.

A. Polyamine btandaxd ( 500 pmole/ml) o& put/ieAcine ( Put), cadavenlne (Cad), apenmidine [Spd), and bpenmine (Spm). B. ed blood ceUU>. C. HomozygouA SS patient, age 26. V. HomozygouA SS patient, age 2. The polyamine c o n t e n t of 500 pmoles of polyamine standard ( p u t r e s c i n e , c a d a v e r i n e , spermidine, and s p e r m i n e ) ( F i g u r e 4A) i s compared with t h a t of normal red blood c e l l s i n Figure 4B, while the polyamine c o n t e n t s of red blood c e l l s from two homozygous s i c k l i n g p a t i e n t s a r e shown in F i g u r e s 4C and 4D.

M. until no further sedimentation occurred. Centrifugation was continued for approximately 30 minutes un­ til all extraneous water that was not tightly bound to the red blood cells had been removed. At this point, the maximum hematocrit value of 74-75% is reached. All subsequent Praxis pulse NMR studies of the resulting solution were computed within one day. Meaou/iejnei'it o£ T\ T\ was measured using a PR103, 10 MHz pulse NMR spectro­ meter with a 90°-90° pulse program. Provided T\ » T2, the free induction following the first 90° pulse decays to zero more rapidly than the magnetization along the z axis reaches its equilibrium value, M 0 .

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Colloid and Interface Science. Biocolloids, Polymers, Monolayers, Membranes, and General Papers by Milton Kerker


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