By E. Heftmann
Chromatography has emerged because the most crucial and flexible analytical technique. The booklet isn't just an up to date model of Heftmann's classical textual content, however it covers parts of destiny significance, equivalent to microfluidics and machine assets. lower than his skilled tips, specialists in every one box have contributed their sensible adventure to an built-in therapy of recent micro research. partially A the theoretical foundation of person separation tools is defined and the technical elements are illustrated. It contains the idea of fuel and liquid chromatography in addition to particular chromatographic strategies, corresponding to size-exclusion, planar, ion, and affinity chromatography in addition to a variety of electrokinetic separation recommendations. Microfluidics are coated for the 1st time and worthwhile resources of analytical tools are indexed and evaluated. 1. each one bankruptcy written via an authority2. Thorough remedy of the theoretical foundation of separation methods3. useful consultant for appearing analyses
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Extra info for Chromatography, Sixth Edition: Fundamentals and applications of chromatography and related differential migration methods - Part A: Fundamentals and technique
35 cm/sec) is substantially smaller than that for gas chromatography (10 cm/sec, Eqn. 49). This is Theory of Chromatography 23 Fig. 6. Plate height vs. linear velocity for a typical open-tubular column for gas chromatography. dc ¼ 200 mm; df ¼ 0:25 mm; DM ¼ 1021 cm2 =sec; DS ¼ 1026 cm2 =sec; k ¼ 3:0: because the optimum velocity is directly related to the diffusion coefficient in the mobile phase, which is four orders of magnitude smaller in liquids than in gases. 050 cm, Eqn. 50), owing to the smaller particle diameter.
3 Optimization methods . . . . . . . . . 1 Simultaneous methods . . . . . . . 2 Sequential methods . . . . . . . 3 Regression methods . . . . . . . 4 Theoretical methods . . . . . . . 4 Univariate optimization . . . . . . . . 1 Temperature . . . . . . . . . 2 Stationary-phase composition . . . . . 3 Mobile-phase composition . . . . . . 4 Other parameters .
3 Regression methods . . . . . . . 4 Theoretical methods . . . . . . . 4 Univariate optimization . . . . . . . . 1 Temperature . . . . . . . . . 2 Stationary-phase composition . . . . . 3 Mobile-phase composition . . . . . . 4 Other parameters . . . . . . . . 5 Multivariate optimization . . . . . . . . 1 Temperature and pressure . . . . . . 2 Temperature and flow-rate . . . . . . 3 Temperature and stationary-phase composition . 4 Temperature and mobile-phase composition .
Chromatography, Sixth Edition: Fundamentals and applications of chromatography and related differential migration methods - Part A: Fundamentals and technique by E. Heftmann