Techniques

Read e-book online Cell Locomotion in Vitro : Techniques and Observations PDF

By C. A. Middleton

ISBN-10: 1461597722

ISBN-13: 9781461597728

ISBN-10: 1461597749

ISBN-13: 9781461597742

It is ten years because the first symposium on mobile locomotion was once held (Locomotion of Tissue Cells, Ciba origin Symposium 14, 1972). That assembly was once chaired by means of Michael Abercrombie, and in his intro­ ductory feedback he commented at the quantity to which the significance of phone locomotion, except that obvious in leucocytes, have been below­ anticipated. a lot has been performed to right that forget throughout the suc­ ceeding decade, and we've discovered extra in regards to the underlying mechanisms of phone locomotion and concerning the components which could impression it. Abercrombie was once himself an incredible contributor to this box of study (as a look on the lists of references during this e-book will confirm), and his principles encouraged the paintings of many different investigators. As in all branches of technology, growth within the research of celliocomo­ tion has relied on the supply of acceptable experimental strategies. of those, tissue tradition has made the best contribution, together with various approaches utilizing both the sunshine or the electron microscope. we've got, hence, tried, in chapters 2 and three, to supply motives of the recommendations which were parti­ cularly fruitful, yet merely in adequate element to allow the reader to isn't a laboratory manual.

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Extra resources for Cell Locomotion in Vitro : Techniques and Observations

Example text

The grids, bearing the now completely dried cells, are then removed, coated with carbon, and placed in the electron microscope. The Preparation of Cultured Cells for Scanning Electron Microscopy For this method of examination, it is particularly important to avoid exposing the living cells to anything which may distort or contaminate their surface. Thus it is advisable to give the cells a preliminary rinse in a balanced salt solution to wash away debris which may have settled onto them from the culture medium, but the saline must be warmed to 37°C and the cells must not be exposed to it for more than a few seconds before being immersed in 2-3 per cent buffered glutaraldehyde, also pre-warmed to 37°C.

A. B. Dreskin (1969) 'An Immunoglobulin-Enzyme Bridge Method for Localizing Tissue Antigens', J. Histochem. , vol. 17, p. A. Middleton (1973) 'The Control of Epithelial Cell Locomotion in Tissue Culture' in Locomotion of Tissue Cells, Ciba Foundation Symposium 14 (Elsevier, Amsterdam), pp. 251-62 C. Milstein (1980) 'Monoclonal Antibodies', Scientific American, vol. 243, p. K. B. Pierce (1966) 'Enzyme-Labelled Antibodies: Preparation and Application for the Localization of Antigens', J. Histochem.

They had been using the cell fusion technique to produce hybrids from a mixture of mouse and rat myeloma cells. Milstein (1980) has described how he and his colleague Kohler conceived the idea that it might be possible to fuse a normal lymphocyte or plasma cell with a myeloma and thus obtain a hybrid which would proliferate indefinitely and whose descendents would all secrete antibody of exactly the same specificity. They chose sheep red blood cells as a test antigen because it is relatively easy to detect antibody against them.

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Cell Locomotion in Vitro : Techniques and Observations by C. A. Middleton


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