Epidemiology

New PDF release: Bridging Laboratory and Field Research for Genetic Control

By B.G.J. Knols, C. Louis

ISBN-10: 1402037996

ISBN-13: 9781402037993

ISBN-10: 1402038003

ISBN-13: 9781402038006

Modern learn on genetic keep an eye on of disease-transmitting bugs is familiar with varieties of scientists: those who paintings within the laboratory and people often called ‘field people’. during the last decade, either teams appear to have built differing study priorities, tackle essentially varied points in the total self-discipline of infectious-disease regulate, and worse, have constructed a systematic ‘language’ that's now not understood by means of the ‘other’ get together. This hole widens each day, among the North and the South, among ecologists and molecular biologists, geneticists and behaviourists, and so on. the necessity to strengthen a standard study time table that bridges this hole has been pointed out as a best precedence via all events concerned. purely then shall the aim of constructing acceptable genetic-control suggestions for vectors of illness turn into fact. This publication is the mirrored image of a workshop, held in Nairobi (Kenya) in July 2004. It introduced jointly an excellent illustration of either molecular and ecological study and, for the 1st time, integrated an important variety of researchers from disease-endemic nations.

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Additional info for Bridging Laboratory and Field Research for Genetic Control of Disease Vectors (Wageningen UR Frontis Series)

Example text

White-Cooper, M. Andreasen and P. Coleman, pers. ). This uses the system known as RIDL (Release of Insects carrying a Dominant Lethal, Thomas et al. 2000). By adding tetracycline to the rearing medium, the construct is ‘switched off’ to allow mass rearing. The effect is genetically dominant so that progeny of released RIDL males mated to wild females will die in the absence of tetracycline in normal breeding sites. A multi-centre trial of mating competitiveness of RIDL males is about to begin in cages.

He therefore proposed the collection of thousands of pupae in the breeding range of one species (Glossina morsitans) and their release into an isolated sector of the breeding range of another (G. swynnertoni) in Tanzania. It was realized that this would only have any useful effect if G. morsitans could compete for mating in the field with G. swynnertoni. Jackson (1945) released both sexes of both species and found that when couples were collected, during their two-hour copulation, all four combinations of the species could be found at frequencies indicating no behavioural barriers to cross-mating.

In the tests with the culicine mosquitoes, males marked with fluorescent powder were released so that the ratio of released sterile to wild fertile males could be determined. Then similarly marked virgin females of wild origin were released, left long enough to mate and then recaptured and made to lay eggs in the laboratory. The proportion of sterile and fertile egg layings was found to be not much less than the sterile:fertile ratio among the males, indicating quite good competitiveness for these females whose marking indicated that they had mated within the village where the males had been released.

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Bridging Laboratory and Field Research for Genetic Control of Disease Vectors (Wageningen UR Frontis Series) by B.G.J. Knols, C. Louis


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