By Professor Dr. Ulrich Winkler, Doz. Dr. Wolfgang Rüger, Priv.-Doz. Dr. Wilfried Wackernagel (auth.)
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Extra info for Bacterial, Phage and Molecular Genetics: An Experimental Course
A small slice of cork, which is placed at the 45 bottom of each centrifuge tube before the gradient is formed, prevents turbulences by the falling drops. After the centrifuge tubes have been filled, carefully remove the slices of cork with a pair of forceps. 15 ml of the sample solution on top of the gradient, using a PASTEUR pipette. Balance the centrifuge tubes of the groups with paraffin and centrifuge overnight (approx. 15 hrs) in an SW56Ti rotor at 30,000 rpm (90,000 x g) at 40 C. Dripping of the gradient (2nd day).
For this purpose express all titers as a fraction (%) of the titer of the control marked zero (0 ml PEG). What was the minimum amount of PEG that removed most of the phages from the upper phase? Estimate the corresponding partition coefficient assuming that the phages which were removed from the upper phase are now in the lower phase. 9 ml PEG-solution? 5) PEG could have been added to the NaCI-containing crude lysate in dry form in order to avoid dilution. How many grams of PEG would have to be dissolved in 10 I crude lysate, if as many phages as possible were to be collected in the interphase?
Nucleic Acids and Transcription Although nucleic acids were isolated and described in the last century (MIESCHER), their key position among cell components has been recognized in only the past 30 years: AVERY and co-workers (1944) proved, by transformation experiments, that deoxyribonucleic acid (DNA) is the carrier of geneti~ information. As life without enzymes and other proteins is impossible, Avery's discovery implied that there must be a connection between DNA and the synthesis of protein.
Bacterial, Phage and Molecular Genetics: An Experimental Course by Professor Dr. Ulrich Winkler, Doz. Dr. Wolfgang Rüger, Priv.-Doz. Dr. Wilfried Wackernagel (auth.)